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Consistent with LaFaive, Fleenor and Nesbit (2008), we employ a linear trend to capture the variation in smoking intensity through time, as indicated in Equation 3: Cons it = Smoke it * f(Trend t ) where f(Trend) represents the above-described linear function of smoking intensity and its systematic under-reporting

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At the end of the experiment, the mice were sacrificed followed by collection of blood via cardiac puncture after anaesthesia