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Miro1 immunohistochemistry Lung antigen retrieval was achieved by submersing slides in a DAKO antigen retrieval solution for 20 min at 95C, slides were then allowed to cool down for 20 min at room temperature and rinsed in three 5 min PBS washes
Vaping, which involves inhaling flavoured vapour, can irritate already-sensitive airways, leading to a prolonged illness or even complications
However, the assessments of substance use relied exclusively on self-report abstinence
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