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The unbalance of interoceptive signals originating from physiological states (the posterior insular cortex) and positive hedonic emotions (the anterior insular cortex) was believed to play a key role in the affective learning of the effects of drugs and their associations with specific contexts [42], which was lined with our findings of abnormal internal connectivity within the insula

When care is taken to avoid the drying of heating coils and/or burning e-liquids in laboratory studies, such as using single puff samples 15 , using power levels described by vapers as popular for specific devices 19 and/or applying settings corresponding to manufacturers recommendations 23 , etc., wide variations in levels of aerosol toxins can still be observed

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