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Its not really surprising concentration per puff is higher, and smokers take more frequent puffs (theres no point in wasting a cigarette, right?)

The slides were then incubated overnight at 4 C with anti-p16 INK4a (1:250, Abcam, Cambridge, UK) in PBS-Triton X-100 (0,3%) and BSA (0,1%), and subsequently for 1 h at room temperature with Alexa 594 conjugated goat anti-rabbit antibody (1:200, Life Technologies, Saint Aubin, France)