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After brushing, the specimens were kept in containers filled with artificial saliva within the incubator at a constant temperature of 37 1 C (the solution of artificial saliva was prepared in the Pharmaceutics Department at the Faculty of Pharmacy, Mansoura University, and contained 23% sodium biphosphate, 11.8% sodium chloride, 11.8% potassium chloride, and 29.5% urea with a pH of 6.8 0.2)