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Due to decay of polonium-210, the annual local dose to localized spots at the bifurcations of segmental bronchi in the lungs caused by smoking cigarettes (1.5 packs/day) is about 80 mSv/year

In vitro harvest for scRNA-seq (WaferGen) For each timepoint, medium was removed from endpoint ALI cultures, and the apical chamber was washed with warm PBS/DTT (10 mM) for 5 min at 37 C, followed by a warm PBS wash of both chambers

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