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It also appeared that tobacco use affects the quantitative mRNA expression of CYP1B1, the induction of which may be influenced by genetic polymorphisms (Helmig et al., 2010)

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Cigarette Stains on Enamel: The Combined Effect The combined effect of nicotine and tar on tooth enamel is what creates the characteristic appearance of cigarette stains on enamel , a layered discoloration that includes both surface tar deposits and deeply embedded nicotine pigmentation