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Libraries were generated from 1 g of total RNA per sample via the NEBNext Ultra RNA Library Prep Kit (New England Biolabs) and then indexed and amplified with Phusion High-Fidelity DNA Polymerase

Karen Knotts on Her Father, Don Knotts: Stories From The Andy Griffith Show and Beyond (EXCLUSIVE) Discover more about Don Knotts, and the abusive childhood he had to overcome to achieve greatness One generation of television watchers knows him as Deputy Sheriff Barney Fife on The Andy Griffith Show

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Further, about 1.3 million deaths and 37.0 million DALYs were associated with second-hand smoke exposure, 50% of which were in India and China or among women