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While the Flavor and Extracts Manufacturers Association considers many flavors to be GRAS in food products, at their levels of intended use, these chemicals could still be harmful when they are aerosolized and inhaled, as such ingredients are not safety tested for exposure routes other than ingestion (Barrington-Trimis et al., 2016

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RT-qPCR RNA isolation, Primer Sequences, RT-PCR on Tfam, OPA1, Drp1, Fis1, Mfn1, Mfn2 using MyiQ single-color Real-Time thermal cycler [30] and RT-PCR for IL-6, IL-8, IL-1, Mn-SOD, PINK1, PPARGC1, OPA1 and TFAM using Taqman were performed as described in the online data Additional file 1

Heated tobacco still contains nicotine and some toxins, but research suggests it exposes users to fewer harmful substances than smoking