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The bronchus was isolated from lobes of lung, minced to small pieces and digested by 0.05% pronase (Sigma, MA, USA) in DMEM/F12 media (Invitrogen, CA, USA) at 4 C overnight

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The Theme of No Smoking Day 2024 The Theme for No Smoking Day 2024 is Protecting Children From Tobacco Industry Interference. National No Smoking Day Timeline 2020 (Still Going Strong): The British Heart Foundations 2020 campaign continues to gain momentum with the slogan, Tobacco breaks your heart. 2003 (Now, Its Official): The Framework Convention on Tobacco Control, which encourages labeled warnings against smoking, is adopted by the World Health Organization