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Subsequently, high-quality and merged sequences were denoised using the DADA2 (Callahan et al., 2016) plugin in the Qiime2 workflow (Bolyen et al., 2019)

Such types of markets in Africa and South-East Asia are difficult to penetrate but can be promising prospects for TTCs, causing them to increasingly become their target of expansion 8

We mention that glass pipes have smooth surfaces, making it easy to maintain hygiene

Because a vape heats liquid rather than burning tobacco, it does not produce the tar and carbon monoxide that cause much of the harm from cigarettes - which is why UK health bodies treat it as substantially less harmful than smoking