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Quantitative Real-Time Polymerase Chain Reaction Total RNA was isolated from the septal nasal mucosa using TRIzol reagent (Life Technologies, Gaithersburg, MD, United States) on day 40 and then converted to cDNA using the ReverTra Ace qPCR RT Master Mix with gDNA Remover (Toyobo, Osaka, Japan) according to the manufacturers instructions