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Moreover, given the paucity of reliable data of nicotine dose, we identified the human-relevant physiological concentration of nicotine by examining the serum level of cotinine, the primary metabolite of nicotine, and a biomarker of tobacco smoke exposure, in mice injected with 2 mg/kg of nicotine in time-dependent manner and found that the cotinine level is comparable with adult smokers serum cotinine level compared to never-smokers as previously described 61 (Supplementary Fig

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