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If youre worried about the increased harshness of throat hit associated with higher nicotine strengths, then wed recommend making the switch to salt nicotine e-liquids which are far smoother on the throat

Brahmachari Sitalprasad, Jain Bauddha TatvaJnana, JainVijay Press, 1934 1 2 3 British Dental Journal

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The Thermocycler (C1000 Thermal cycler, Bio-Rad, USA) was used following this program: Initial denaturation at 95 C for 3 min followed by 35 cycles of denaturation at 95 C for 30 s