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The primary antibody was prepared using 4 l of the given antibody solution (p-Erk, p p-38, p-JNK, p38, JNK, ERK and Actin) and 4 ml of a 2% BSA (Bovine Serum Albumin) Solution

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In order to translate observed breathing patterns and smoking behavior into biological effects, we combined the small airway-on-a-chip with a smoking machine that burns cigarettes and a microrespirator that inhales and exhales small volumes of cigarette smoke and fresh air in and out of the epithelium-lined channel in programmable intervals mimicking true smoking behavior, said Richard Novak, a co-author of the study and a senior staff engineer at the Wyss Institute, who helped design the smoking instrument

population smoked, and most of the other half accepted the practice as socially acceptable