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Sussan TE, Shahzad FG, Tabassum E, et al

A 5 ml mixture containing 1666.7ul of each LDH assay substrate solution, LDH assay cofactor, and LDH assay dye solution was prepared, of which 50 L (standard kit volume) was pipetted into each well containing samples (Figure 2)

The Department of Public Health further clarifies that e-cigarettes are battery-powered, noncombustible tobacco products designed to deliver nicotine and other substances to users by creating an aerosol that simulates smoking