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All experiments were performed in 3 biological and 6 technical replicates (18 wells per group) and a total of 864 wells were analyzed

The loophole requires urgent attention given the UK has thus far succeeded in relatively low youth uptake of e-cigarettes, especially among those who have never smoked.10 The success may be attributable to careful regulation, which included an early ban on advertising that could cross borders and sale of the products to children.11 Thirdly, it may draw vapers away from refillable e-cigarettes towards pod devices.12 As refillable e-cigarettes can be filled with any generic brand of e-liquid, manufacturers cannot make profit by offering these devices for free or selling them at a loss