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However, as discussed in section 3.1, there are species differences associated with many physiological functions, and as such effects observed in animal models do not always reflect the mechanisms involved in human responses

Steeping time depends on the tea type and brewing method: Black tea: 35 minutes for hot brew, 812 hours for cold brew Green tea: 23 minutes for hot brew, 68 hours for cold brew Herbal tea: 510 minutes for hot brew, 812 hours for cold brew Avoid over-steeping, as it can make iced tea taste bitter or overly astringent

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