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catechu ) control samples to test identification potential of key compounds including hydroxychavicol (associated with piper betle leaf), arecoline (a biomarker for betel nut), and nicotine (associated with tobacco, a modern betel nut additive) through liquid chromatography-mass spectrometry (LC-MS) analysis, and (2) Analysis of 36 archaeological dental calculus samples from six human burials from Nong Ratchawat, Central Thailand [4,000 BP (4,0803,850 cal BP