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After the administration of a local anesthetic, an incision was performed at the level of the interscapular zone, to subcutaneously implant an osmotic minipump (Model 2004, ALZET, CA, USA) containing 200 L of either a solution of nicotine hydrogen tartrate salt (Sigma-Aldrich, USA) at a dose of 10 mg/kg/day (free base) or saline solution (H 2 O with 0.9% NaCl) for the control group