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RT-qPCR RNA isolation, Primer Sequences, RT-PCR on Tfam, OPA1, Drp1, Fis1, Mfn1, Mfn2 using MyiQ single-color Real-Time thermal cycler [30] and RT-PCR for IL-6, IL-8, IL-1, Mn-SOD, PINK1, PPARGC1, OPA1 and TFAM using Taqman were performed as described in the online data Additional file 1

Some people have slower-acting versions of CYP2A6, which results in nicotine staying in their system for a longer time, while others have more efficient variants that break down nicotine more quickly

Pros and cons of vaping Vaping can help people give up smoking, and vaping is much less harmful