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Because of Tolkien, cultural understanding of classically imaginary creatures like elves and trolls has evolved and advanced

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Subsequently, PCR was used to amplify the variable region of the fungal ITS region ITS1 using the primer sequences ITS1F (5-CTTGGTCATTTAGAGGAAGTAA-3) and ITS2R (5-GCTGCGTTCTTCATCGATGC-3