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T cell activation Fresh whole blood samples collected in heparin were diluted tenfold with RPMI and 90 l of the diluted blood was incubated in 96-well flat-bottom tissue culture plates in a humidified incubator at 5% CO 2 , 37C for 2 days in the presence or absence of anti-CD3 + anti-CD28

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In reality, a relatively small proportion of counties (though representing a disproportionately large share of the population) experienced statistically significant declines over this period