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The third and fourth leaves were harvested and ground in liquid nitrogen at 2, 3 and 4 d post-inoculation, and the total RNA was extracted using the FastPure Universal Plant Total RNA Isolation Kit (China) and reverse transcribed in cDNA

It showed significant over-segmentation and under-segmentation in certain areas, with a CPA of only 77.85%, Recall of 81.58%, IoU of 66.22%, F1 score of 79.37%, and Dice score of 85.78%

Retailers such as convenience stores or smoke shops typically add a 10-15% markup on packs, which equates to about $1 to $2 a pack

Its common sense that if its illegal to sell these products to kids, it should be illegal for kids to have them, said Morrison in an email