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The threshold intensity was manually set and kept constant, and the number of pixels was determined for 4G8-, ThS-, Iba1- and GFAP stained sections to quantify amyloid load, fibrillar burden, microglial activation and reactive astrogliosis in hippocampal (CA1, CA2, CA3 and dentate gyrus), subiculum and cortical areas (including motor, somatosensory, visual, frontal, parietal and retrosplenial cortex)

Survey results, published in Tobacco Control , show that less than 5% of the 3,500 adult e-cig users who responded to the survey quit using e-cigs in response to the flavored e-cig ban

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