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Do not use gasoline lighters or cardboard matches, as the smoke from them has an odor and can spoil the taste of the cigar
The conditions of qPCR were as follows: an initial denaturation at 95 C for 5 min, followed by 40 cycles of 95 C for 30 s, 55 C for 30 s, and 72 C for 30 s, concluding with a final extension at 72 C for 5 min
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