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Each behavioral test was recorded and an observer blinded to the experimental conditions measured overall distance traveled and time spent in the center zone from observation of the digital recordings ( The LD apparatus consists of two connected compartments (40 cm 40 cm 30 cm), one of which is illuminated (light/aversive area) and the other of which is dark (dark/safe area), with an opening in the wall separating them (7.5 cm 5 cm) ( 2.5 Brain tissue harvesting After the final behavioral test, rats were euthanized using diethyl ether, decapitated with a guillotine and their brains were immediately removed and stored at 80C until processing

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