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In a randomized crossover trial comparing healthy subjects who smoked tobacco (maximum of 10 cigarettes per month), the acute use of nicotine-containing e-cigarettes significantly increased levels of circulating endothelial progenitor cells (flow cytometry) to the same magnitude as after smoking a traditional cigarette (41)

Classification of E3 ubiquitin ligases E3 ubiquitin ligases determine the specificity of ubiquitin attachment to substrate proteins and are considered critical components of the UPS