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The images were acquired by a Leica TCS SP8 X confocal microscope (Leica Microsystems, Germany) and the fluorescence intensity quantification was performed using a FiJi software 14

In the case of cigarette smoking, nicotine has a detrimental effect on insulin action, and it appears that e-cigarettes may also have the same effect. But this isnt the first time smoking has been linked to developing prediabetes and type 2 diabetes

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