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Primary antibodies against B-cell lymphoma-2 (Bcl-2) (Cell Signaling Technology, USA, 1:1000), Bcl-2 Associated X (BAX) (Abcam, USA, 1:1000), Cleaved-caspase 3 (Cell Signaling Technology, USA, 1:1000), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Abcam, USA, 1:1000) were incubated with PVDF membranes overnight at 4C

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