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Briefly, binary masks of Synaptophysin and PLA positive regions were obtained by subtracting the background (rolling ball radius 10 pixels), applying a 1 sigma radius Gaussian blur filter on Synaptophysin signal and setting a user-defined threshold

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However, it has been structurally modified to bind to albumin in the bloodstream, which dramatically extends its half-life to approximately six to eight days (with DAC formulation)

However, when combined with GLP-1 activation, glucagon effects shift dramatically toward beneficial metabolic outcomes