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Tissues were blocked in CAS block (Thermo Fisher Scientific) and incubated overnight with primary antibodies diluted in CAS block (Table 1): Primary antibodies for immunofluorescence Slides were washed 3 times in PBST, incubated overnight with secondary antibodies, and diluted in PBS/BSA 1% (Table 2): Secondary antibodies for immunofluorescence Slides were then washed once in PBS and closed with cover glass using ImmuGio mounting medium (DiaSorin, 2505-5)

Methods: A retrospective cohort study using data from the TriNetX US Collaborative Network, covering 66 healthcare organizations from May 2022 to April 2024

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(1980) Effects of low level administration of dichlorvos on adreno- corticotrophic hormone secretion, adrenal cholesteryl ester, and steroid metabolism