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The HiLoad 16/600 Superdex 75 column (GE Healthcare) was used to separate the monomeric fractions of Nb35 with running buffer containing 20 mM HEPES, pH 7.5 and 100 mM NaCl

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Of note, glutathione peroxidase 4 (GPX4)-deficient cells in the erythroid lineage were found to be more prone to RIPK3-mediated necroptosis, which was found to be ROS-dependent

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