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Writing Team for the Diabetes Control and Complications Trial/Epidemiology of Diabetes Interventions and Complications Research Group (2002) Effect of intensive therapy on the microvascular complications of type 1 diabetes mellitus

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The separation was carried out on a Welch Ultisil Amino Acid (4.6250 mm, 5 m) column under a gradient program using sodium acetate trihydrate solution: acetonitrile (93:7) as mobile phase A and acetonitrile: water (80:20) as mobile phase B

Cholecalciferol is hydroxylated in the liver into 25-hydroxycholecalciferol, which is further hydroxylated in the kidney into 1,25-dihydroxycholecalciferol (active form)