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Following initial monoplex trials, the primers were pooled in the two tubes and multiplex PCR was optimized using human reference DNA in conjunction with ONT sequencing

This is usually beneficial yet may become problematic if too many are present in a formula or when they are paired with potent blood-thinning plant extracts

People with kidney stones related to high uric acid levels are typically advised to avoid purine-rich foods (UMMC 2013), but a low-animal protein or vegetarian diet that alkalinizes the urine is more important for preventing uric acid stone formation (Kenny 2010