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Key risk factors include: Obesity and central adiposity particularly visceral fat accumulation Type 2 diabetes mellitus insulin resistance is a central pathophysiological mechanism Dyslipidaemia elevated triglycerides and low HDL cholesterol Hypertension part of the metabolic syndrome cluster Sedentary lifestyle lack of physical activity contributes to metabolic dysfunction The pathogenesis involves a 'multiple-hit' hypothesis, where insulin resistance leads to increased hepatic lipogenesis and reduced fatty acid oxidation, whilst oxidative stress, inflammatory cytokines, and gut-derived endotoxins contribute to hepatocellular injury and fibrosis

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To enhance embryonic nutrition beyond what is naturally deposited in the egg, in ovo injection technology has been developed as a strategic tool for supplying exogenous nutrients directly to the developing embryo 9,10