doi: 10.3390/nu14173540
The amplification was performed by using TaqMan gene expression assays (Hs00167309, Hs00156308, Hs01110250, Hs00195591, Hs01566408, Hs04989912, Hs00958111, Hs04189111, Hs00426835, and Hs99999905) according to the manufacturers instructions
As Arg615 is involved in multiple interactions with BSol residues, one likely explanation for the gain-of-function of R615C is that it destabilizes the closed state relative to the open state of the channel, resulting in a gain-of-function phenotype, manifesting as an enhanced sensitivity to activating ligands, including atorvastatin
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