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On the experimental day, cells were washed twice and placed into KRB buffer (132.2 mM NaCl, 3.6 mM KCl, 5 mM NaHCO 3 , 0.5 mM NaH 2 PO 4 , 0.5 mM MgCl 2 , 1.5 mM CaCl 2 and 0.001 g/mL BSA, pH 7.4) supplemented with 25 mM glucose (1 h, 37 C, 5% CO 2 )

After preliminary functional screening (see the following paragraphs), N -hexanoic-Tyr-Ile-(6) amino-hexanoic amide was chosen for further investigation with the expectation that it would be biologically active, metabolically stable, and BBB-permeable

Mezcla tcnica: Inyecte 2 ml de agua BAC lentamente por la pared interna del vial

Moderate Stage Dementia is characterized by, for example, short term memory loss