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For the measurement of cAMP, cAMP was extracted from frozen intestine tissue samples by lysis buffer and analyzed by a mouse cAMP Assay kit (ab138880, Abcam)

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Multiple studies suggested that PCOS may further induce serious complications such as diabetes, hypertension, endometrial cancer (EC) and preeclampsia without timely intervention, which can severely affect the quality of life and long-term health outcomes of patients ( Current clinical practice recognizes that there is no one-size-fits-all therapeutic approach for PCOS management

Morning: 1-2 tablespoons of chia seeds in water or a smoothie (providing roughly 10 grams of fiber)