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Braak H, Alafuzoff I, Arzberger T, Kretzschmar H, Tredici K

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The reaction was monitored at an absorbance of 600 nm for 2 min with a Biotek Epoch 2 spectrophotometer

Much of this increase is explained by changes in diagnostic practice, including: Broadening the definition of ASD to include higher-functioning children Increased accuracy in the diagnosis of ASD (particularly for children who previously may have been diagnosed with intellectual disability) Increased awareness of the disorder Earlier identification of young children However, we cannot rule out that there is a true increase in the risk for ASD