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The columns were washed with 2 1 ml BSA solution, which was also collected, and then, the suspension was centrifuged at 300 g for 5 min at 4C

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However, mitochondrial dysfunction can lead to a buildup of ROS due to an imbalance in their production and degradation 4,5 Another major source of ROS is NADPH oxidase (Nox), a membrane-bound enzyme complex that produces superoxide by transferring an electron to oxygen from NADPH

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