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Western blot analysis Total proteins were extracted from pancreatic or cells in RIPA lysis buffer (Applygen, C1055) containing a 1% protease inhibitor cocktail and 1 mM PMSF

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It has a strong reputation for promoting: Tendon and ligament repair Muscle and joint recovery Gut healing and ulcer repair Brain and nerve regeneration Reduced inflammation and pain It works by triggering growth factors like VEGF (Vascular Endothelial Growth Factor), accelerating angiogenesis (formation of new blood vessels), and improving collagen synthesis

That discovery provided the genetic blueprint for a synthetic version called exenatide, marketed as Byetta, which was approved in 2005 as a treatment for type 2 diabetes. Ozempic was developed as a newer synthetic drug that mimics GLP-1, inspired by the discovery of exendin-4 in Gila Monster saliva