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The mass spectrum with positive ionization mode for complex 2 after 1 min of incubation showed two signals of the iron(III) complex at m/z = 254 and at m/z = 376, corresponding to the [M L] + and [M + Na] + species

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The 5-formimino-THF is then converted to 5-10-methenyl-THF via the action of the cyclodeaminase activity of the complex with glutamate and ammonia being the other products