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Similarly, inhibiting glutathione reductase (GR), which converts oxidized glutathione (GSSG) to GSH, with 2-AAPA induced oxidative stress, leading to the suppression of melanoma metastasis in vitro and in vivo [33]

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Indeed, chronic ROS can cause protein oxidation of methionine and cysteine residues 112,113 , which in the absence of GSH becomes permanent and might decrease irreversibly CI and CII functionality