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Tomar 1 mL del extracto en un tubo de ensayo de 15 mL, aadir 9 mL de Acetonitrilo, mezclar en vortex durante 1 min, luego tomar una parte y colocarla en un tubo de centrifugacin de 5 mL, centrifugar a 16000 r/min durante 5 min, el sobrenadante obtenido se reserva para la purificacin

] Functional Synergies of the Blend Angiogenesis and Perfusion