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On the next day, the membranes were washed and incubated with secondary antibodies (Proteintech, China) for 1 h at room temperature

Ringer Solution Sensitizes Erythrocytes to Eryptosis We assessed the impact of the media itself (without eryptosis inducers and Albumax supplementation) on eryptosis and observed that erythrocytes exposed more PS and displayed increased hemolysis when incubated in Ringer when compared to iRPMI ( Figures 2C, D )