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HBV infection was validated by the presence of HBsAg and HBV DNA in the serum and HBV RNA in the liver

The purified DNA was digested into component nucleotides using nuclease P 1 , snake venom phosphodiesterase and alkaline phosphatase as previously described in detail

This information enables us to elucidate the effects of individual mutations and provide us with the genotype-phenotype correlation ( + binding site may cause more detrimental effects compared to mutations of residues in other less conserved sites

NAD+ does more than produce energy - it acts as a co-substrate for enzymes such as sirtuins and PARPs that maintain DNA repair and genomic stability