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Cells were harvested by centrifugation, resuspended in phosphate-buffered saline (PBS) buffer (140 mM NaCl, 2.7 mM KCl, 10 mM NaHPO, 1.8 mM KHPO, pH 7.4, and 10% glycerol), and lysed by sonication

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CBS and CSE, both of which are pyridoxal 5-phosphate (PLP)-dependent enzymes, function sequentially in the transsulfuration pathway, converting homocysteine and serine into cystathionine and subsequently cysteine (Figures 2 a,b) ( 2 S by utilizing alternative substrates, namely, a combination of cystine and homocysteine for CBS and either cysteine or homocysteine for CSE ( in vitro (Figure 2c) ( in vivo

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