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For the purification of His-tagged AtSNX1 and His-tagged AtSNX2a, the procedure was the same as that for full-length AtSNX1, except for slight variations in buffer compositions

Nitric oxide (NO) is a free radical gas molecule produced endogenously from L-arginine, oxygen, and NADPH by an enzyme nitric oxide synthase (NOS) [139]

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The methionine and choline-deficient diet (commonly applied in mice and rats) and choline-deficient L-amino-defined diet rapidly induce NASH, but fail to reproduce the pathophysiological response corresponding to clinical observations [125, 126]