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In HFD-fed mice, oral administration of 100 g P9* reduced weight gain, food intake, adipose tissue volume and glucose intolerance and stimulated Gcg expression in the ileum

Understanding both helps explain why the type of food matters not just whether food was consumed at all

As the primary objective was hypothesis-generating rather than confirmatory, randomization or blinding procedures were not implemented

The assay was conducted in the wells of 96-well black plastic microplates, in which 20 L of enzyme solution and 60 L of bile salt were mixed together, and then 20 L of substrate was added, with mixing, at the start of the reaction, which proceeded at room temperature for up to 30 min, with measurement at various intermediate time points